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How-tos

Running a Baby EEG experiment: checklist

Last updated on 6 August 2021 by Ty Mees

If you have suggestions on how to improve this document, or find mistakes, please send them to labman.gw@uu.nl

This is a general checklist for both infant EEG experiments. For more the adult version of the checklist, please see Running an EEG experiment: Piloting your Experiment.

Preparation

In the control room:

  • Turn on  the Recording and the Presentation computers, and turn on all the screens.
    • In K.13: the screens are turned on by turning on the ‘Main Power’ plugbox (on the right of the desk).
  • Select an infant movie to play (if applicable).
    • In K.13: Set the video switch to the correct screen.
  • Turn on the speakers
    • In K.02: (in the cabin) turn on the speakers you want by turning on the corresponding plug box in the cabin.
    • In K.13: Turn on the desired amplifier and set the audio switch to corresponding  amplifier
  • Before the participant is in the lab, run the experiment once to check whether sound and visual stimuli work well.

In the test room:

  • Check the battery power; if the battery is somewhat empty it’s recommended you switch out the battery with the spare one in the control room (which should be full).
    • You can check the battery level through ActiView, or by connecting it to the charger. If the charger gives you a green light, it’s good to go. If it’s orange, you should only use it if the other battery is empty as well.
  • Connect the battery to the amplifier (EEG and ABR experiments use different modules/amplifier): connect 4 sides. Lift the black connector slightly until you hear a click, then close gently. Finally connect the battery to the amplifier with the blue connector in the front.
  • Put gel into syringe
  • Get one EEG cap (there’s a size-chart in the closet containing the caps)
  • Get the electrodes bundle (32 Channel)
  • Turn on the air humidifier (only in K.13)
  • Check the sound level using the audiometer. A loudness of 65 dB is recommended for the sound stimuli.
  • Put the sound absorbing towel on top of the table
  • Put the toys on the table (if applicable)
  • Optional: prepare the cap in advance

Receive the participant

For infant experiments:

  • Welcome the parent and the baby in the waiting area (K.01). Take them to the lab
  • Switch off your mobile phone and make sure the participant does the same.
  • Have the participant remove any jewelry (e.g. necklaces or earrings).
  • Put a little bit of gel on the baby’s wrist.
  • Explain to the parent what the experiment is about and what is expected of them/what they can expect. Tell the parents:
    • Don’t let the baby pull the hat or the electrodes.
    • Don’t talk during the experiment.
    • If the baby gets fuzzy, you can stop at any time.
  • Check the baby’s wrist for allergic reactions. If absent, continue with the experiment.

Start the experiment

In the test room:

  • Clean the skin behind the participant’s ear with a cotton pad with alcohol.
  • Put EXG1 on the left mastoid and EXG2 on the right mastoid, connect these two electrodes to the amplifier in the drawer.
  • Put on the cap.
  • Put gel into all electrodes.
  • Connect electrodes to the corresponding holes in the cap and to the amplifier.
  • Turn on the amplifier and check the blue light.

In the control room:

  • Click the BioSemi icon on the Recording PC desktop in the control room.
  • Click “start” on the BioSemi window, you should see all the electrodes now.
  • Check impedance: go to tab “electrode offset”, set the scale on the left to 50mv, and all the blue bars should be in the range ±25mv.
  • Set reference to Cz: go to the tab “monopolar display”. On the left of the screen, click “Free choice” under “Reference”, and use the menu below to select Cz.
  • Click “Start File” at the right bottom of the BioSemi window.
  • Type in the participant’s information and save in your data folder.
  • Click “pause save”, otherwise you are not recording!
  • Open OBS Studio and click ‘Start recording’.
  • On Presentation PC, open the experiment file, click run.
  • Fill in the name of the participant in the pop up window “Enter Subject Name/ID”, in the same format as in ActiView (Recording PC).
  • Use Enter key on the keyboard to start experiment. Use Esc if you want to abort the experiment.
  • On the Recording PC, check whether triggers are sent. If not, call lab support.

After the experiment (infants)

  • Ask if the parent wants to wash their baby’s head.
  • Collect consent form from the parent.
  • Give the baby the gift (if applicable).
  • Reimburse travel expenses.
  • Ask for remaining questions.

Cleaning

  • Clean the cap and electrodes
  • Always use lukewarm water
  • Never let electrodes sit under running water!
  • Put away the toys and the cushion (if applicable)
  • Fill in the experiment report